| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Deciphering the molecular adaptation of the king scallop (Pecten maximus) to heat stress using transcriptomics and proteomics
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| Autores: |
Artigaud, Sebastien
Richard, Joelle
Thorne, Michael A. S.
Lavaud, Romain
Flye-sainte-marie, Jonathan
Jean, Fred
Peck, Lloyd S.
Clark, Melody S.
Pichereau, Vianney
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| Data: |
2015-11
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| Ano: |
2015
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| Palavras-chave: |
Marine biology
Metabolism
DNA repair
Transcription regulation
Apoptosis
Energy reserves
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| Resumo: |
Background: The capacity of marine species to survive chronic heat stress underpins their ability to survive warming oceans as a result of climate change. In this study RNA-Seq and 2-DE proteomics were employed to decipher the molecular response of the sub-tidal bivalve Pecten maximus, to elevated temperatures. Results: Individuals were maintained at three different temperatures (15, 21 and 25 degrees C) for 56 days, representing control conditions, maximum environmental temperature and extreme warming, with individuals sampled at seven time points. The scallops thrived at 21 degrees C, but suffered a reduction in condition at 25 degrees C. RNA-Seq analyses produced 26,064 assembled contigs, of which 531 were differentially expressed, with putative annotation assigned to 177 transcripts. The proteomic approach identified 24 differentially expressed proteins, with nine identified by mass spectrometry. Network analysis of these results indicated a pivotal role for GAPDH and AP1 signalling pathways. Data also suggested a remodelling of the cell structure, as revealed by the differential expression of genes involved in the cytoskeleton and cell membrane and a reduction in DNA repair. They also indicated the diversion of energetic metabolism towards the mobilization of lipid energy reserves to fuel the increased metabolic rate at the higher temperature. Conclusions: This work provides preliminary insights into the response of P. maximus to chronic heat stress and provides a basis for future studies examining the tipping points and energetic trade-offs of scallop culture in warming oceans.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00301/41173/40360.pdf
DOI:10.1186/s12864-015-2132-x
https://archimer.ifremer.fr/doc/00301/41173/
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| Editor: |
Biomed Central Ltd
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| Formato: |
application/pdf
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| Fonte: |
Bmc Genomics (1471-2164) (Biomed Central Ltd), 2015-11 , Vol. 16 , N. 988 , P. 1-14
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| Direitos: |
2015 Artigaud et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommo
info:eu-repo/semantics/openAccess
restricted use
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